Application of a PCR Workflow for Sensitive and Specific Mycoplasma Detection in Biologics
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Mycoplasma contamination remains a persistent and often underrecognized concern in cell culture and biologics production. These microorganisms are challenging to detect using routine microscopy due to their extremely small size and lack of a cell wall, which allows them to evade conventional detection methods. The presence of mycoplasma can significantly impact cell physiology, alter protein expression profiles, interfere with cellular metabolism, and ultimately compromise the reproducibility and reliability of both research outcomes and biopharmaceutical products.